The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn't have a solid background in sequencing, she keeps asking "simple" - even silly at times - questions and Daniel gives his best to make us understand.
Iuliia and Daniel have fun with salt water and alcohol Chapters: 00:00:00 DIY DNA isolation 00:15:38 Directed evolution of Enzymes DNA isolation in your kitchen - Experiment during the episode: https://www.instructables.com/How-to-Extract-Your-Own-DNA-Using-Household-Kitche/ - Another protocol with the same approach: https://learning-center.homesciencetools.com/article/how-to-extract-dna-at-home/ - Extract DNA from a strawberry: https://www.popsci.com/diy/diy-science-project-strawberrry-dna/ - Banana DNA extraction: https://askabiologist.asu.edu/activities/banana-dna BONUS: Directed, artifical evolution of enzymes: - Where it comes from: https://www.nobelprize.org/prizes/chemistry/2018/arnold/facts/ - Chemistryworld about the idea: https://www.chemistryworld.com/news/what-is-directed-evolution-and-why-did-it-win-the-chemistry-nobel-prize/3009584.article - A few of many results of this method: https://pdb101.rcsb.org/motm/228 - MIT News about adding robots to this: https://news.mit.edu/2021/robotic-directed-evolution-molecules-1230 Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany. Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ). The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
Erschienen: 04.08.2023
Dauer: 00:22:47
Weitere Informationen zur Episode "Special Episode 1: Kitchen Genomics - DIY DNA Isolation"
Daniel and Iuliia are confused about fireflies - Scientific american on how fireflies create light: https://www.scientificamerican.com/article/how-and-why-do-fireflies/ - A small breakdown of a typical Pyrosequencing workflow: https://www.news-medical.net/life-sciences/What-is-Pyrosequencing.aspx - Biology-pages on Pyrosequencing: https://www.biology-pages.info/P/Pyrosequencing.html - Biotechfront on Pyrosequencing: https://www.biotechfront.com/2021/04/pyrosequencing-principle-and-steps.html - Shomu's Biology on Pyrosequencing: https://www.youtube.com/watch?v=he6kJStE2zs - Quick Biochemistry Basics explaining the method: https://www.youtube.com/watch?v=wY8to-_zAEo - Bionity on Pyrosequencing: https://www.bionity.com/en/encyclopedia/454_Life_Sciences.html Chapters: 1:00 Intro 3:00 Fireflies 4:00 Energy 6:00 Luciferase Reaction 11:00 TTTTTTTTTTTTTTT...T? 14:00 microwell Plates 18:00 Pyrosequencing 25:00 Storage & Price 30:00 Teaser 31:00 Pyro? 34:00 Outro Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ). The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
Erschienen: 14.07.2023
Dauer: 00:34:24
Weitere Informationen zur Episode "Episode 2: Pyrosequencing"
Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotide Chapters: 00:00-Intro 02:00-DNA 08:27-Enzymes 13:05-RNAs, tRNAs 16:18-mRNAs, µRNAs, lncRNAs, circRNAs 21:35-Wobble 23:30-PCR 33:20-Sanger sequencing I 36:30-Gel electrophoresis 40:00-Sanger sequencing II 53:10-Teaser next Episode, Outro Topics: DNA, RNA, Protein, PCR, Gel Electrophoresis, Sanger Sequencing - The Royal Institution on the DNA Discovery: https://www.youtube.com/watch?v=_w2453iom9s - The discovery of DNA on science history: https://sciencehistory.org/education/scientific-biographies/james-watson-francis-crick-maurice-wilkins-and-rosalind-franklin/ - The New York Times on Franklins’ part: https://www.nytimes.com/2023/04/25/science/rosalind-franklin-dna.html - Wikipedias article on Proteins with history: https://en.wikipedia.org/wiki/Protein - Jrank on RNA: https://science.jrank.org/pages/5871/Ribonucleic-Acid-RNA-discovery-RNA.html - Microbe Notes showing the central dogma of molecular biology: https://microbenotes.com/central-dogma-replication-transcription-translation/ - ThoughtCo on RNA and DNA: https://www.thoughtco.com/dna-versus-rna-608191 - khanacademy explainers on PCR and Gel electrophoresis: https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/polymerase-chain-reaction-pcr - Amoeba Sisters on PCR: https://www.youtube.com/watch?v=a5jmdh9AnS4 - Promega explaining Sanger sequencing: https://www.youtube.com/watch?v=dVRB4CaLizc - Microbe online’s writeup on Sanger sequencing: https://microbeonline.com/dna-sequencing-sanger-sequencing-method/ - LibreTextsBiology on Proteins and Enzymes: https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Book%3A_General_Biology_(Boundless)/03%3A_Biological_Macromolecules/3.07%3A_Proteins_-_Types_and_Functions_of_Proteins - excedr on Taq Polymerase and PCR: https://www.excedr.com/resources/taq-polymerase-what-is-it-and-what-does-it-do/ Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ). The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903). P.S.: One DNA nucleotide is ~0.34 nanometers in „length“ - DR
Erschienen: 26.06.2023
Dauer: 00:53:49
Weitere Informationen zur Episode "Episode 1: Basics - from DNA to Sanger sequencing"