Podcast: Active Motif's Podcast
Erschienen: 10.02.2022
Dauer: 28:26
In this episode of the Epigenetics Podcast, we caught up with Yad Ghavi-Helm from the Institut de Génomique Fonctionnelle de Lyon to talk about her work on enhancer-promoter interactions during mammalian development. The Laboratory of Yad Ghavi-Helm focuses on how developmental genes are regulated by enhancers. They could show that developmental genes are often regulated by more than one enhancer and that those enhancer can often be located many kilobases away on the linear chromosome. Furthermore, their work also indicates that the interaction of promoters and their respective enhancers are usually established before the expression of the target gene is switched on and that those interactions are generally stable during embryogenesis. In addition, those stable interactions seem to coincide with paused RNA Pol II being located at those promoters before gene activation. References Ghavi-Helm, Y., Michaut, M., Acker, J., Aude, J.-C., Thuriaux, P., Werner, M., & Soutourina, J. (2008). Genome-wide location analysis reveals a role of TFIIS in RNA polymerase III transcription. Genes & Development, 22(14), 1934–1947. https://doi.org/10.1101/gad.471908 Ghavi-Helm, Y., Klein, F. A., Pakozdi, T., Ciglar, L., Noordermeer, D., Huber, W., & Furlong, E. E. M. (2014). Enhancer loops appear stable during development and are associated with paused polymerase. Nature, 512(7512), 96–100. https://doi.org/10.1038/nature13417 Ghavi-Helm, Y., Jankowski, A., Meiers, S., Viales, R. R., Korbel, J. O., & Furlong, E. E. M. (2019). Highly rearranged chromosomes reveal uncoupling between genome topology and gene expression. Nature Genetics, 51(8), 1272–1282. https://doi.org/10.1038/s41588-019-0462-3 Related Episodes Ultraconserved Enhancers and Enhancer Redundancy (Diane Dickel) Unraveling Mechanisms of Chromosome Formation (Job Dekker) Biophysical Modeling of 3-D Genome Organization (Leonid Mirny) Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com
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