The open-source DIY Sequencer was here.Update: 454.bio is now dead, but the archives still have it: https://web.archive.org/web/20250723191639/https://454.bio/Their GitHub:https://github.com/454bioElements new VITARI: https://www.elementbiosciences.com/products/preorder-vitari
Erschienen: 07.04.2026
Dauer: 00:25:52
We have the same thing at home!- yes, but in worse, cheaper or bothQitan:https://pmc.ncbi.nlm.nih.gov/articles/PMC8985760/https://www.qitantech.com/The QPinnacle2: https://www.vcbeathealth.com/article/1821CycloneSEQ, the MGI-internal clone:https://en.cyclone-seq.com/nanopTechhttps://www.prnewswire.com/news-releases/mgi-launches-new-nanopore-sequencing-products-with-advanced-cycloneseq-technology-302241816.htmlhttps://www.biorxiv.org/content/10.1101/2024.08.19.608720v1https://rrwick.github.io/2024/12/17/cycloneseq.htmlhttps://gigabytejournal.com/articles/154https://gigasciencejournal.com/blog/open-cycloneseq-benchmarking-for-complete-bacterial-genomes/Polyseq:https://polyseq.com/Axbio:https://www.axbio.cn/products/45.htmlGeneus-tech:http://www.geneus-tech.com/proddetail.aspx?id=1Nice not up-to-date table of specs:https://github.com/zhangtianyuan666/NanoporeReview2024ONT to double promethion throughput: https://www.genomeweb.com/sequencing/jpm-oxford-nanopore-shares-plans-double-flow-cell-output-expand-menu-biopharma-market
Erschienen: 20.03.2026
Dauer: 00:38:46
Weitere Informationen zur Episode "Special: other Nanopore Sequencing"
Artificial Intelligence everywhere, and sometimes its useful.Correction: apparently the Biostar Handbook is not free anymore :(AlphaGenome:https://omicsomics.blogspot.com/2026/02/non-coding-dnas-alpha-moment.htmlhttps://www.alphagenomedocs.com/installation.htmlhttps://www.genomeweb.com/informatics/google-alphagenome-users-scrutinize-ai-models-ability-predict-variant-effectshttps://deepmind.google.com/science/alphagenome/Bioinformatics Forums:Bioinformatics mistakes on Biostars: https://www.biostars.org/p/7126/Bioinformatics stackexchange:https://bioinformatics.stackexchange.com/Seqanswers:https://www.seqanswers.com/forum/sequencing-technologies-companiesVibecoding (current setup, top to bottom):https://claude.ai/newhttps://gemini.google.com/apphttps://duck.ai/If danger is something you enjoy, use this: https://openclaw.ai/YOU HAVE BEEN WARNEDPaper about old LLMs that can solve simple, old and solved bioinformatics problems:https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1011511Youtube has useful bioinformatics courses:1: https://www.youtube.com/watch?v=3XFpxQF0J74&list=PLWVKUEZ25V95IKyBrxHtRTK_6Ig8Xi9-f2: https://www.youtube.com/watch?v=LvtPXZHNoCc&list=PLeB-Dlq-v6taAXK6ZCGfqImrNWJzFt3p3
Erschienen: 10.03.2026
Dauer: 00:35:57
Weitere Informationen zur Episode "News: AlphaGenome, Vibecoding"
When do you get your Illumina sticker for your ONSO? This is the first part of a two-parts news roundup.PacBio News:https://aseq.substack.com/p/pacbio-sells-short-read-tech-to-illuminahttps://www.pacb.com/press_releases/pacbio-completes-sale-of-short-read-sequencing-assets/https://albertvilella.substack.com/p/pacbio-sells-short-read-ip-to-illuminaThe 6-base sequencing run:https://www.pacb.com/wp-content/uploads/PAG-5hmC-Portik.pdfhttps://www.nature.com/articles/s42003-025-07681-0ONT's P2 solo drama:https://omicsomics.blogspot.com/2026/01/p2-solo-ont-extends-support-but-not.html
Erschienen: 20.02.2026
Dauer: 00:24:33
For when matching and sticking is enough to get
Erschienen: 23.12.2025
Dauer: 00:41:34
After 454 now the second try by Roche.Also:PacBio makes sequencing cheaper: https://www.pacb.com/press_releases/pacbio-announces-major-advances-for-revio-and-vega-to-lower-genome-cost-and-expand-multiomic-capabilities/Roche about SBX: https://sequencing.roche.com/global/en/article-listing/sequencing-platform-technologies.htmlRoche from a conference: https://www.youtube.com/watch?v=YN2meswiCiQSince 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: https://ngs-cn.de/explain-podcast/
Erschienen: 18.11.2025
Dauer: 00:42:50
Weitere Informationen zur Episode "Sequencing by Expansion (SBX)"
This time something different.Sources:https://www.23andme.com/privacy/https://verbraucherschutzforum.berlin/2025-03-25/23andme-meldet-insolvenz-an-was-nutzer-jetzt-wissen-sollten-357738/https://en.wikipedia.org/wiki/23andMe_data_leakhttps://www.cshub.com/attacks/news/23andme-hacker-leaks-datahttps://www.cbsnews.com/news/dna-privacy-at-home-tests-23andme-ancestrydna-sell-data-to-third-parties/https://techcrunch.com/2025/03/24/23andme-faces-an-uncertain-future-so-does-your-genetic-data/https://www.theverge.com/2024/9/13/24243986/23andme-settlement-dna-data-breach-lawsuithttps://newsroom.regeneron.com/news-releases/news-release-details/regeneron-enters-asset-purchase-agreement-acquire-23andmer-256/
Erschienen: 02.10.2025
Dauer: 00:21:59
The science of white blood cells, and much moreChapters:05:30 Immunology how?22:05 22q11.2 deletion syndrome34:30 Scientific collaboration40:00 Data losses50:00 Flip it!53:00 outlook 2030Links:mini-immunology intro:https://www.youtube.com/watch?v=k9QAyP3bYmc22Q e. V. - zentraler Anlaufpunkt für Menschen mit Deletionssyndrom 22q11 oder Duplikationssyndrom 22q11 im deutschsprachigen Raum https://www.wirsind22q.de/aus-dem-verein/vorstandPodcast for young immunologists from young immunologists https://theyoungimmunologist.buzzsprout.com/Contact Nora here https://www.linkedin.com/in/nora-balzer-phd-253a767bSome information about training courses and events https://www.immunology.org/traininghttps://dgfi.org/dgfi-en/academy-of-immunology/Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: https://ngs-cn.de/explain-podcast/
Erschienen: 06.08.2025
Dauer: 00:57:00
Sharing a flowcell, reinventedChapters:- 00:00 usegalaxy.org- 07:55 Singular Genomics start- 21:55 Max Reads- 29:40 DNA Structure / ATAC-seqSetup galaxy on local hardware: https://galaxyproject.org/admin/get-galaxy/G4 is competing with Illumina:https://www.genengnews.com/topics/omics/fast-and-flexible-singulars-g4-takes-aim-at-illuminas-sequencing-superiority/Singulars chemistry is one of many:https://www.nature.com/articles/d41586-023-00512-4How max reads works:https://techwriting.singulargenomics.com/max-read/Max-Read-Kit-User-Guide-600026.pdfSmall intro to DNA Structures:https://en.wikipedia.org/wiki/Nucleic_acid_quaternary_structureIntro to ATAC-seq:https://www.nature.com/articles/s41596-022-00692-9PS:As of 2025, Singular does not advertise DNA/RNA Sequencing on their G4 anymore, but shifted to spatial data, including their G4X: https://www.singulargenomics.com/Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: https://ngs-cn.de/explain-podcast/
Erschienen: 28.05.2025
Dauer: 00:46:07
Weitere Informationen zur Episode "E15: Singular Genomics | ATAC-seq"
Spinnin' bases on wafers to do mostly natural SBSChapters:- 00:00 Ultima ePCR with beads- 07:10 Spinning the wafer, nucleotides, no blocking- 14:30 Ultima ppmSeq + Element UltraQ- 25:30 How Element UltraQ was born- 30:30 snakemake, cromwell, nextflow- 36:50 ncRNAUltimas homepage: https://www.ultimagenomics.com/Nature paper with the chemistry: https://www.nature.com/articles/s41587-022-01452-6Elements UltraQ chemistry:https://www.elementbiosciences.com/cloudbreak-ultraq-enhanced-sequencing-accuracy-when-precision-matters-mostSnakemake: https://snakemake.readthedocs.io/en/stable/Nextflow: https://www.nextflow.io/docs/latest/index.htmlCromwell: https://cromwell.readthedocs.io/en/stable/Small overview of not all ncRNA types: https://microbenotes.com/types-of-rna/Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).
Erschienen: 02.04.2025
Dauer: 00:46:20
Weitere Informationen zur Episode "Episode 14: Ultima Genomics + Element UltraQ"
